MARN331 Marine Phytoplankton Ecology
and Physiology—Fall 2004
3 credits. Prerequisite: BIO107, 108, MARN170,
MARN260/380 or consent of instructor.
Instructor: Senjie Lin
Phytoplankton diversity and distribution,
photosynthesis and growth in relation to genetic and environmental
factors, cell death, top-down control, and current topics in
phytoplankton research. Methodology of measurements will be an
Lecture: Wed 9:45-11:30 am, Room #123.
Lab: Fri 9:30 am-12:30 pm, Room #107.
None. The class will be largely based on literature.
materials: assigned literature materials (selected papers from
the Reading List).
Grading: final grade is determined by
points from final exam (45%), lab performance (30%), and term paper
(Aug 30, Sep 3)
Objectives & structure
Assignment of term papers
Lab 1: Culture isolation
Week 2 (Sep 8, Sep 10)
Species diversity and distribution
Species diversity and challenges in
Phytoplankton biomass (chlorophylls, carbon,
Epifluorescence microscopy and flow cytometry
Identification of phytoplankton (lab cultures and field samples from
Long Island Sound).
(Sep 15, 17)
Chloroplast diversity and pigment ratios
Evolution: endosymbiosis and gene transfer
Ecological variants: Structure, pigments,
Chlorophylls, chlorophyll to carbon ratios
(Sep 22, 24)
4: Photosynthesis and effects of light
Photochemistry and biochemistry in
P-I functional response.
Light adaptation and photoinhibition.
Effects of UV light.
Long Island Sound Cruise.
(Sep 29, Oct 1)
Reproduction and life history.
Cell division cycle.
Lab 5: Chlorophyll (cruise sample) and
Week 6 (Oct 6, 8)
Effects of nutrients I
Requirement of N, P, Si, trace metals
Sources and abundances.
Lab 6: P vs I curve
Week 7 (Oct 13, 15)
Lecture 7: Effects of nutrients II
Lab 7: P vs I curve.
Term paper 1st draft due
Week 8 (Oct 20, 22)
Lecture 8: Iron limitation
Requirement of iron, cobalt, zinc, etc.
Symptoms of iron limitation and adaptation.
Consequence of iron fertilization.
Lab 8: Measurement of nitrate reduactase
(Oct 27, 29)
Lecture 9: Heterotrophy in planktonic microalgae
mixotrophic the norm?
Lab 9: Measurement of nitrate reduactase activity.
Week 10 (Nov 3, 5)
division rate (gross growth rate)
Lab 10: Dilution experiment
Week 11 (Nov 10, 12)
Lecture 11: Growth and
growth rate and population dynamics
death: “altruistic suicide”?
Lab 11: Dilution
Week 12 (Nov 17, 19)
Lecture 12: Production and
biomass: Remote sensing technology
- Measurement methods
- Total and system-specific productions
- Biological pump and its efficiency
- Positive & negative feedbacks
Lab 12: 14C method
Week 13 (Nov. 22-28)
Thanksgiving Recess: No class
Week 14 (Dec 1, Dec 3)
Lecture 13: Community structure & function in response to
dominated system (Si, NO3 vs. NH4)
dominated system (oligotrophic)
dominated system (CaCO3 formation)
stress: Coral-Symbiodinium complex
Lab 13: 14C method
(Dec 8, 10)
Final Exam (take home exam, distributed on Dec 8,
due back Dec 15)
Term paper: due on Dec 17.