Mitochondrial cytochrome b (cob) and cytochrome C oxidase subunit I (cox1) in dinoflagellates
Mitochondrial cytochrome b gene has widely been used for phylogenetic analysis, population genetic structure analysis, and species identification for wide range of organisms. However, it has not been studied in dinoflagellates. Recently, we isolated this gene from the dinoflagellate Pfiesteria piscicida and examined its utility for phylogenetic analysis and species identification. Three copies of this gene were found, one of which appeared to be pseudogene. Neighbor-joining trees based on nt sequences exhibit more even pace of evolution across biological taxa than 18S rDNA. Based on the sequence, primers were designed one set of which was shown to be highly specific to P. piscicida. A Time-Step PCR protocol was established to quantify cell concentration of this species from both laboratory cultures and field-collected plankton samples. Appl. Environ. Microbiol. 68 (2002): 989-994.